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af1997 nestin goat igg  (R&D Systems)


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    R&D Systems af1997 nestin goat igg
    Af1997 Nestin Goat Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/af1997 nestin goat igg/product/R&D Systems
    Average 95 stars, based on 465 article reviews
    af1997 nestin goat igg - by Bioz Stars, 2026-03
    95/100 stars

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    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), <t>NANOG</t> (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .
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    Primary and secondary antibodies used for ICC

    Journal: Fluids and Barriers of the CNS

    Article Title: Human iPSC-derived pericyte-like cells carrying APP Swedish mutation overproduce beta-amyloid and induce cerebral amyloid angiopathy-like changes

    doi: 10.1186/s12987-024-00576-y

    Figure Lengend Snippet: Primary and secondary antibodies used for ICC

    Article Snippet: Goat anti-Nanog , 1:100 , R&D Systems , AF1997.

    Techniques:

    a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: a) Immunocytochemistry for the hiPSC related markers, TRA-1-60 (upper panel, green), NANOG (upper panel, blue), SOX2 (lower panel, green), and OCT3/4 (lower panel, red) performed on expanded clone. b) Karyostat assay (ThermoFisher) demonstrating no abnormalities detected. The whole genome view displays all somatic and sex chromosomes in one frame with high level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number. Referent to .

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Immunocytochemistry, Microarray

    a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: a) Images of clinical grade OSCs (lot 90) grown on laminin on day 5 of hiPSC expansion and day 5 of OSC differentiation. Scale bar, 250 μm. b) Flow cytometry analysis of 4 markers: FOXL2, CD82, OCT4, and NANOG. Expression levels of these markers were tested against a control and CG-OSC-L. d) UMAP projection of the CG-OSC subset. e) UMAP projection of the individual lots found in the CG-OSC-L subset. f) Stacked bar plot depicting the amount of each cluster type found in each lot relative to the CG-OSC-L subset. Overall percentages per group are given to the right of the barplot. g) Dotplot representing the expression of granulosa cell markers in the CG-OSC subset. Scale represents ‘Mean expression in groups’ ranging from 0 to 2, and circles represent ‘Fraction of cells in group (%) ranging from 0 to 100. h) GO chord plot for differently regulated proteins in both RUO-OSC and CG-OSC versus hiPSC. i) Correlation curve for proteins detected in the secretome of RUO-OSC versus CG-OSC.

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Flow Cytometry, Expressing, Control

    Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .

    Journal: bioRxiv

    Article Title: Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

    doi: 10.1101/2024.04.29.591741

    Figure Lengend Snippet: Bar plots depicting relative expression of hiPSC related markers, OCT4 and NANOG in OSCs after 5 days of differentiation (CG-OSCs). Clinical-grade (CG)-hiPSC are used as a positive control. Expression is normalized by expression of the housekeeping gene, GAPDH. Referent to .

    Article Snippet: The primary antibodies used were mouse monoclonal antibody against OCT3/4 (1:200; sc5279, Santa Cruz Biotechnology), goat polyclonal antibody against SOX2 (1:50; AF2018, R&D systems), goat polyclonal antibody against NANOG (1:50; AF1997, R&D systems), and Alexa Fluor 488 mouse monoclonal antibody against TRA-1-60 (1:100; 560173, BD Biosciences).

    Techniques: Expressing, Positive Control